Research Facilities and Resources
This facility, located in the university’s Department of Pharmacology, houses two instruments: a gas chromatograph/mass spectrometer (GC/MS), Hewlett-Packard 5989 Mass Engine; and a liquid chromatography/mass spectrometer (LC/MS) system, Esquire LC/MS Brucker-Daltonics.
Routine quantitative GC/MS analyses are performed using negative ion chemical ionization mode, an autoinjector and a specialized data system. In addition to electron and chemical ionizations, mass spectrometric analyses can be performed by fast atom bombardment, particle beam or direct inlet probes.
The new Esquire LC/MS electrospray instrument is particularly useful for analyses of biomolecules of high molecular weight and polarity that cannot be analyzed by GS/MS. Several stages of mass spectrometry assist in the advanced structural identification of complex biomolecules such as proteins, peptides, phospholipids and glucuronides. The Esquire LC/MS can detect negative and positive ions and has advanced computerization and chromatographic capabilities.
At the university, the new instrumentation is being used to improve understanding of the role of eicosanoids and the interaction of peroxynitrite with macromolecules in health and disease. The GC/MS instrument is used primarily for quantitative and structural analyses of arachidonic acid metabolites such as HETEs, epoxides and prostaglandins.
Contact:Michael Balazy, Ph.D.
Professor of Pharmacology
New York Medical College
Department of Pharmacology
Basic Sciences Building
Valhalla, NY 10595
Tel: 914/594-4291
Fax: 914/594-3621
E-Mail: michael_balazy@nymc.edu
