Flow cytometry allows rapid analysis and classification of cells or other small particles. Fluorescence-activated cell sorting (FACS) enables researchers to sort live or fixed cells rapidly according to their physical characteristics or cell surface markers so that subpopulations of cells can be studied separately. These powerful techniques have broad research and clinical applications. Research fields such as immunophenotyping, cell cycle, apoptosis, cell signaling, and cell sorting/isolation are well established areas of application of these techniques. Of particular interest is the current application of flow cytometry and sorting to identify and isolate stem cells. New applications of flow cytometry are still being developed constantly.
The Cytometry Core Laboratory of New York Medical College is located in the Brander Cancer Research Institute, a 4,300 sq. ft. research facility in the Department of Pathology. The laboratory is equipped with a Beckman Coulter MoFlo XDP high-speed flow cytometer/sorter equipped with three laser light sources for simultaneous excitation in the UV, blue and red. The system can acquire two light scatter parameters (forward and right angle) and eleven fluorescence measurements from violet (405 nm) to near infrared (785 nm). The instrument is equipped with four way sorting and the ability to sort single cells into multiwell plates under sterile conditions. Also available is a Becton Dickinson FACScan flow cytometer for three-color analysis.
The laboratory is also equipped with a CompuCyte four laser (violet, blue, yellow and red) iCys scanning cytometer for four color analysis of fluorescent stained cells on slides as well as ratios of the various parameters. The instrument operates at a rate up to a hundred cells per second and has the capacity to revisit cells, record cell images, measure live and fixed cells on the slide and create a photomontage of selected cells.
Computerized flow cytometry data acquisition and analysis systems coupled with most up-to-date software packages (FlowJo, Kaluza, and Multicycle) are available in the laboratory.
The Brander Cancer Research Institute is an international leader in using flow cytometry techniques to study the action of antitumor drug, basic research into molecular control of cell proliferation, apoptosis and the DNA damage response pathways. BiomedExperts ranked number 1 on cytometry worldwide.
The laboratory provides high quality comprehensive services on flow cytometry and FACS analyses. These include project consultation, sample assay, and data analysis. The laboratory is also eager to help investigators to explore potential new applications that may be of special value in their research projects.
Investigators considering flow cytometry service should contact the laboratory for consultations to device suitable experimental plans. Several hundred of specific protocols describing applications of cytometry for particular research projects, published in the Current Protocols in Cytometry, are also available to the users of the core laboratory to plan their experiments. Users from the NYMC community will enjoy favorable pricing.
Jiangwei Li, Ph.D.
Technical Supervisor, Flow Cytometry Core Laboratory
Dazhong Xu, Ph.D.
Head, Flow Cytometry Core Laboratory
Zbigniew Darzynkiewicz, M.D., Ph.D.
Director of Brander Cancer Institute
Brander Cancer Research Institute
Department of Pathology
New York Medical College
Basic Science Building, Room 438
Valhalla, NY 10595