NYMC > Research > Core Facilities & Shared Resources > Lipidomics and Mass Spectrometry

Lipidomics and Mass Spectrometry Core

The Lipidomics & Mass Spectrometry Core is located in the Department of Pharmacology and provides support for faculty and student researchers both here at New York Medical College and at other institutions and private companies.  Our staff provides a wide range of expertise and will work with you during the planning and protocol development stages to ensure excellent results. We can also provide figures to assist in manuscript preparation.

The LC-MS/MS allows for quantification and identification of lipid mediators from biological matrices as it is highly selective and sensitive without the need for time consuming derivation.  Our ultra-sensitive instrument is equipped with a UPLC and auto-sampler (Shimadzu LC-MS 8050). We are able to run substantial amounts of samples overnight due to our gas generators from PEAK Scientific and Parker Balston.  Sensitivity differs among analytes and depends on sample matrix; however, we generally can detect 0.1 to 1 pg of standards on column.

We can detect both free and esterified fatty acids (after a hydrolysis step) Our current list of lipid mediators include the arachidonic acid (AA)-derived eicosanoids, docosahexaenoic acid (DHA) and eicosapentaenoic (EPA)-derived resolvins, protectins, and maresins also collectively called specialized pro-resolving mediators (SPMs).  See full list below.  We are constantly updating this list and can design specialized custom methods for almost any fatty acid.

All users please acknowledge in your publications that these samples were analyzed by The Shimadzu LCMS-8050 in the Lipidomics and Mass Spectrometry Core at New York Medical College.

These services are available as a fee for service.  The cost per sample is determined by the sample work-up necessary and the number of analytes you wish to quantify.  Please e-mail us for current pricing.

Contact:

Lars Bellner, Ph.D.
Instructor, Pharmacology

LCMS Core Lab Manager
Tel:  914-594-4939
E-mail:  lars_bellner@nymc.edu

Michal L. Schwartzman, Ph.D.
Professor and Chair, Pharmacology
LCMS Core Director
Tel:  914-594-3116
E-mail:  Michal_Schwartzman@nymc.edu

 

Compounds

[M-H]-

Cyclooxygenase Products

 

PGF2a

353

8-iso PGF2a

353

PGD2

351

PGE2

351

Lipoxygenase Eicosanoids

 

LXA4

351

LXB4

351

5,15-diHETE

335

5,12-diHETE

335

12-OxoETE

317

5-OxoETE

317

12-HETrE

321

15-HETrE

321

15-HETE

319

12-HETE

319

5-HETE

319

Specialized Pro-resolving Mediators (SPMs)

 

Protectin D1

359

Maresin 1

359

Maresin 2

359

Resolvin D5

359

Resolvin D1

375

Resolvin D2

375

Resolvin D3

375

17-HDHA

343

14-HDHA

343

13-HDHA

343

7-HDHA

343

4-HDHA

343

CYP450 Eicosanoids

 

14,15-DiHETrE

337

11,12-DiHETrE

337

8,9-DiHETrE

337

5,6-DiHETrE

337

14(15)-EET

319

11(12)-EET

319

8(9)-EET

319

5(6)-EET

319

20-HETE

319

19-HETE

319

18-HETE

319