NYMC > Research > Core Facilities & Shared Resources > Lipidomics and Mass Spectrometry

Lipidomics and Mass Spectrometry

The Lipidomics & Mass Spectrometry Core is located in the Department of Pharmacology and provides support for faculty and student researchers both here at New York Medical College and at other institutions and private companies.  Our staff provides a wide range of expertise and will work with you during the planning and protocol development stages to ensure excellent results. We can also provide figures to assist in manuscript preparation.

The LC-MS/MS allows for quantification and identification of lipid mediators from biological matrices as it is both highly selective and sensitive without the need for time consuming derivation.  Our newest ultra sensitive instrument, a Shimadzu LC-8050, is equipped with a UPLC and auto-sampler (LC-MS 8050).  Other instruments include an AB Sciex 3200 Qtrap system which is equipped with an Agilent HPLC and auto-sampler (3200-Qtrap); this model is sensitive enough for most applications.  We are able to run large amount of samples overnight because of our  gas generators from PEAK Scientific Parker Balston.  Sensitivity differs between analytes and depends on sample matrix; however we generally can detect 0.1 to 1 pg of standards on column.

We can detect both free and esterified fatty acids (after a hydrolysis step) Our current list of lipid mediators include the arachidonic acid (AA)-derived eicosanoids, docosahexaenoic acid (DHA) and eicosapentaenoic (EPA)-derived resolvins, protectins, and maresins also collectively called specialized pro-resolving mediators (SPMs).  See full list here (link to table below).  We are constantly updating this list and can design specialized customs methods for almost any fatty acid.

The GC/MS system (Agilent 6890N/5973) has been set up for carbon monoxide (CO) analyses in samples from in vitro and in vivo studies.

All users please acknowledge NIH grant support for the Lipidomics & Mass Spectrometry Core in their publications with the following text:  “This study was supported in part by National Institutes of Health Grant HL 034300”.

Both the LC-MS/MS and GC/MS services are available as a fee for service.  The cost per sample depends on sample work-up needed and how many analytes you wish to quantify.  Please e-mail us for current pricing.

Contact:

Katherine Gotlinger
Core Lab Manager
Tel:  914-594-4939
E-mail:  katherine_gotlinger@nymc.edu

Michal L. Schwartzman, Ph.D.
Professor and Chair, Pharmacology
New York Medical College
E-mail:  michal_schwartzman@nymc.edu

 

Compounds

[M-H]

Cyclooxygenase Products

 

PGF2a

333

8-iso PGF2a

353

PG D2

351

PG E2

351

Lipoxygenase Eicosanoids

 

LXA4

351

LXB4

351

5,15-diHETE

335

5,12-diHETE

335

12-OxoETE

335

5-OxoETE

317

12-HETrE

321

15-HETrE

321

15-HETE

319

12-HETE

319

5-HETE

319

Specialized Pro-resolving Mediators(SPMs)

 

Protectin D1

359

Maresin 1

359

Maresin 2

359

Resolvin D5

359

Resolvin D1

375

Resolvin D2

375

Resolvin D3

375

17-HDHA

343

14-HDHA

343

13-HDHA

343

7-HDHA

343

4-HDHA

343

 

 

CYP450 Eicosanoids

 

14,15-DiHETrE

337

11,12-DiHETrE

337

8,9-DiHETrE

337

5,6-DiHETrE

337

14(15)-EET

319

11(12)-EET

319

8(9)-EET

319

5(6)-EET

319

20-HETE

319

19-HETE

319

18-HETE

319